Table 3.
Image analysis quantification of immunohistochemistry for mGluR1 and mGluR5 in dorsal horn of animals treated with mGluR1 oligonucleotides
| Gray scale intensity (arbitrary units) in excess of dorsal column control | ||||
|---|---|---|---|---|
| Control | mGluR1sense | mGluR1 mismatch | mGluR1 antisense | |
| mGluR1 | ||||
| Lamina I | 96.5 ± 1.1 | 95.4 ± 2.0 | 94.8 ± 1.5 | 86.8 ± 2.9 |
| Lamina II0 | 98.6 ± 0.6 | 97.2 ± 1.6 | 95.3 ± 1.3 | 84.5 ± 3.4*,***,**** |
| Lamina III | 80.3 ± 2.7 | 70.5 ± 5.0 | 75.0 ± 1.7 | 53.1 ± 3.3**,***,***** |
| Lamina III | 23.3 ± 3.0 | 20.3 ± 2.2 | 25.9 ± 2.2 | 16.5 ± 4.8 |
| Ventral horn | 26.3 ± 2.0 | 22.7 ± 1.8 | 28.0 ± 6.3 | 25.7 ± 1.0 |
| mGluR5 | ||||
| Lamina I | 70.6 ± 2.8 | 82.3 ± 2.5 | 77.2 ± 2.5 | 80.4 ± 2.8 |
| Lamina II0 | 66.7 ± 2.8 | 73.5 ± 2.9 | 58.3 ± 3.8 | 71.8 ± 4.5 |
| Lamina III | 43.3 ± 3.3 | 47.4 ± 3.0 | 36.9 ± 2.8 | 43.9 ± 3.7 |
| Lamina III | 17.9 ± 2.5 | 13.7 ± 2.3 | 17.4 ± 3.0 | 13.8 ± 2.4 |
| Ventral horn | 32.0 ± 1.3 | 28.5 ± 2.3 | 26.7 ± 2.7 | 30.4 ± 2.0 |
The statistically significant differences were determined by Mann–Whitney U test (n = 15–16). *p < 0.05, **p < 0.01 compared with control; ***p < 0.05 compared with sense; and ****p < 0.05, *****p < 0.01 compared with mismatch.