Skip to main content
. 1998 Dec 1;18(23):9766–9779. doi: 10.1523/JNEUROSCI.18-23-09766.1998

Fig. 3.

Fig. 3.

Guinea pig PACAP is homologous to other mammalian forms. Guinea pig cardiac ganglia cDNA was amplified with the oligonucleotide primer pairs PCPX1/PCPX2 and PCPX5/PCPX6. The products were purified, blunt-ended, and ligated into the pCR-Script cloning vector for transformation into competent cells. Automated fluorescence dideoxy dye terminator sequencing was performed in both directions using the T3 and T7 primer sites. The nucleotide sequence for the region of the guinea pig (cavia parcillus) transcript encoding the mature PACAP peptides is aligned with analogous regions of the rat [rattus norvegicus; Ogi et al. (1990); GenBankM63006], mouse [mus musculus; Okazaki et al. (1995); GenBank D14716], sheep (ovis aries; Ohkubo et al. (1992); GenBank S83511], and human [homo sapiens; Ohkubo et al. (1992); GenBank S83513) sequences. Nucleotides that differ from the guinea pig sequence are shown in white; regions of identity are shaded. The predicted amino acid sequences for the PACAP peptides are shown below. All base substitutions were degenerate, resulting in the conservation of all the amino acid residues encoding the PACAP peptides among species.