PAC1 receptor protein immunoreactivity is localized to cardiac ganglia neuronal plasma membranes. A, B, Dissociated cardiac ganglia neurons were immunocytochemically processed for PAC1 receptor (Cy3, red) and MAP-2 (FITC,green) and imaged under confocal microscopy. Plasma membrane labeling for the PAC1 receptor circumscribed the MAP-2-positive cardiac neurons. Scale bar, 50 μm.