Fig. 5.

Semiquantitative analysis of the pCREB-IR induced by PACAP (A) or glutamate (B) in mouse SCN brain slices. A, PACAP application (100 nm) evoked a robust pCREB-IR in the SCN of C3H and C57BL mice when applied at ZT10 but had no effect at ZT14, ZT18, or ZT06. B, Glutamate application (100 μm) induced pCREB-IR in the SCN of C3H and C57BL mice at ZT14 and ZT18 but had only slight effects at ZT06 and ZT10. Untreated slices (controls) showed a very low basal pCREB-IR (see also Fig. 7). Each data point represents the mean ± SEM of four to nine animals. Asterisks indicate significantly different values of stimulated slices compared with controls; *p < 0.05; ***p < 0.001.