Fig. 2.
Effect of DA on the phosphorylation of NR1. Nucleus accumbens slices, which had been prelabeled with [32P]phosphate for 60 min, were incubated for 0.5–10 min with dopamine (100 μm) plus nomifensine (10 μm), a dopamine uptake inhibitor. [32P]phosphate incorporation into NR1 was determined as described in Materials and Methods. The data were calculated as percentage of radioactivity in control slices and represent means ± SEM for five (0–5 min) or three (10 min) experiments (different from control; *p < 0.05, **p < 0.01; Student’s ttest).