Fig. 3.
Effect of D1-type and D2-type dopamine agonists on the state of NR1 phosphorylation. Nucleus accumbens slices, which had been prelabeled with [32P]phosphate for 60 min, were incubated for 2.5–10 min with a D1 agonist (SKF82526, 1 μm) or a D2 agonist (quinpirole, 1 μm), as shown in autoradiograms (A) and a bar graph (B). [32P]phosphate incorporation into NR1 was determined as described in Materials and Methods. Data were calculated as percentage of radioactivity in control slices and represent means ± SEM for four experiments (different from control; *p < 0.05, **p < 0.01; Student’s t test).