Fig. 4.

Effect of calyculin A, a protein phosphatase-1/2A inhibitor, on the phosphorylation state of NR1. Nucleus accumbens slices, which had been prelabeled with [³²P]phosphate, were incubated for 5–45 min in the absence or presence of calyculin A (200 nm). [³²P]phosphate-labeled NR1 was analyzed as described in Materials and Methods. Data were normalized to radioactivity in slices incubated for 45 min with calyculin A and represent means ± SEM for four experiments (different from control; *p < 0.05; Mann–Whitney Utest). Incubation of slices in the absence of calyculin A for 45 min had no effect on the amount of [³²P]phosphate-labeled NR1.