Fig. 6.

Effect of genetic deletion of DARPP-32 on the state of phosphorylation of NR1. Nucleus accumbens slices prepared from wild-type and DARPP-32 mutant mice were prelabeled with [³²P]phosphate for 60 min and then incubated in the absence or presence of DA (100 μm) plus nomifensine (10 μm) for 1 min (A), forskolin (1 μm) for 5 min (B), or PDBu (5 μm) for 15 min (C). [³²P]phosphate incorporation into NR1 was determined as described in Materials and Methods. Data were calculated as percentage of radioactivity in control slices and represent means ± SEM for three to six experiments (different from wild type; * p < 0.05; Student’s ttest).