Fig. 8.

Enhancement of the survival of Purkinje cells by rhM-CSF. Samples rich in Purkinje cells were prepared from cerebellar cell suspensions of E18; then the cells were cultured in a serum-free medium with or without rhM-CSF for 7 d. As a control group, Purkinje cells that had been cultured in serum-containing medium were used. The cells were then processed for immunostaining with anti-calbindin antibody to calculate the ratio of survival of the Purkinje cells compared with the control group. Each value represents the mean and 1 SD. Statistical analysis was performed by one-way ANOVA with Scheffé’s multiple comparison procedure (significance withp < 0.05). The control group differs from every other group. The groups with asterisks do not differ from each other, nor do the nonmarked groups, but in all other comparisons the differences are significant.