Fig. 5.

Mitochondrial depolarization is still neuroprotective in the presence of elevated cytoplasmic Ca²⁺. Cells were incubated in the presence of 5 μg/ml oligomycin and 2.6 mm external Ca²⁺ and exposed to 100 μmglutamate–10 μm glycine for the indicated period in the absence (A) or presence (B) of 2 μm rotenone. Where indicated, 1 μmMK-801 was added. C, D, Frequency histograms of [Ca²⁺]_c observed at 60 min in individual somata from a minimum of four independent experiments under the same conditions in the absence (C) or presence (D) of 2 μmrotenone.