Fig. 6.

ICD under conditions of energy limitation: conditions for reversal. Granule cells were incubated fromt = 0 in the presence of 1 μmcarbonyl cyanide p-trifluoromethoxyphenyl hydrazone (A), 1 μm antimycin A (B), or 2 μm rotenone (C–F). At 2 min, 100 μmglutamate–10 μm glycine were added. Where indicated, 5 μg/ml oligomycin (oligo) and/or 1 μmMK-801 and 2 mm Mg²⁺ were added. Note that NMDA receptor inhibition per se does not restore Ca²⁺ homeostasis. Representative experiments from a minimum of three independent experiments are shown. Values in theinsets represent the proportion of cells (mean ± SEM) showing recovery of [Ca²⁺]_c to <500 nm after 60 min of amino acid exposure.