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. 1998 Dec 15;18(24):10398–10408. doi: 10.1523/JNEUROSCI.18-24-10398.1998

Fig. 5.

Fig. 5.

The heteromeric association of Kv1.2 and Kv1.5 channel α subunits in sciatic nerve. Reciprocal coimmunoprecipitation of Kv1.5 and Kv1.2 in P4 sciatic nerve is shown. A, Homogenates of acutely isolated sciatic nerves from P4 mice were subjected to immunoprecipitation with anti-Kv1.2, anti-Kv1.5, anti-Kv3.1, and preimmune antibodies. The Kv1.2 and Kv1.5 subunits were depleted from sciatic nerve extracts with their respective antibodies, and unbound proteins were subjected to a second round of immunoprecipitation with anti-Kv1.2 (Kv1.5 depl. Kv1.2) and anti-Kv1.5 (Kv1.2 depl. Kv1.5), respectively. Blots were probed with anti-Kv1.5. B, Reciprocal coimmunoprecipitation of Kv1.2 with Kv1.5 in P4 sciatic nerve. The blot shown in A was stripped and reprobed with anti-Kv1.2.