Fig. 3.

Western blot analysis of the induction of theArc protein in the hippocampal formation by ECS. Rats received a single electroconvulsive stimulus and were killed at varying times postseizure. Hippocampi were dissected and sonicated into boiling SDS sample buffer. Homogenates were subjected to Western analysis withArc-specific antibody. A clear increase inArc protein was observed by 30 min, and the highest protein level was seen at 4 hr. Levels of Arc protein returned to the pre-seizure baseline by 24 hr. The blot was probed with an antibody against α-CaMKII to control for equivalency of protein loading.