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. 1998 Jan 1;18(1):26–35. doi: 10.1523/JNEUROSCI.18-01-00026.1998

Fig. 5.

Fig. 5.

Newly synthesized NGFI-A and ArcmRNA are sorted to their appropriate compartment after inhibition of protein synthesis. A–D, Sheet film autoradiograms of a hemibrain composed of the left hemisphere of an animal 2 hr post-ECS and the right hemisphere of a 2 hr post-ECS animal pretreated with cycloheximide (20 mg/kg) 15 min before ECS. Expression of NGFI-A mRNA was superinduced by ECS in the presence of cycloheximide (B) relative to ECS alone (A) but remained restricted to the granule cell layer. Arc mRNA showed the normal pattern of dendritic translocation (C) despite pretreatment with cycloheximide (D). Scale bar, 2.5 mm.Insets, Higher magnification of the hippocampal formation from A–D. Scale bar, 500 μm. E, F, Nonradioactive in situ hybridization of adjacent sections to compare the cellular distribution of NGFI-A (E) and Arc (F) mRNA 2 hr after ECS in an animal pretreated with cycloheximide. The maintained localization of NGFI-A mRNA over dentate granule cell bodies indicates that mRNA does not diffuse freely in dendrites when it is not part of a translational complex. The migration of ArcmRNA throughout the dendritic lamina therefore is consistent with its selective transport. SG, Stratum granulosa. Scale bar, 50 μm.