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Immunofluorescence photomicrographs of sections of the arcuate nucleus (Arc) after direct double labeling combining antiserum to LR (A, C) with antiserum to neuropeptide Y (NPY) (B, D). LR- and NPY-positive neurons are located mainly in the ventromedial part of the Arc. Double labeling shows that LR-LI is present in many NPY-containing neurons (arrows). ME, Median eminence; VMN, ventromedial nucleus;3v, third ventricle. Scale bars, 50 μm.
