Skip to main content
. 1998 Jan 1;18(1):237–250. doi: 10.1523/JNEUROSCI.18-01-00237.1998

Fig. 8.

Fig. 8.

Binding site requirements for cooperativity between Sox10 and Tst-1/Oct6/SCIP. A, The POU-responsive luciferase reporter plasmid 3xHSVoct luc was transfected into U138 glioblastoma cells in combination with empty CMV expression plasmid (−), pCMV/Sox10 (0.2 μg/plate; Sox10), and pCMV/Tst-1 (0.2 μg/plate; Tst-1) as indicated. Luciferase activities were determined in three independent experiments, each performed in duplicate. Values from transfections with luciferase reporter and empty expression plasmid were arbitrarily set to 1. Data from all other transfections are presented as fold induction above this level. B, Purified Sox10-GST protein (Sox10) and nuclear extracts from COS cells transfected with Tst-1/Oct6/SCIP (Tst-1) were analyzed in electrophoretic mobility shift assays for their ability to bind to a radiolabeled HSVoct oligonucleotide, which contained the binding site for POU domain proteins from the HSV ICP0 promoter, as shown at thetop. The Tst-1/Oct6/SCIP-specific complex is marked by an arrowhead.