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. 1998 Jan 1;18(1):128–137. doi: 10.1523/JNEUROSCI.18-01-00128.1998

Fig. 1.

Fig. 1.

Syntrophin, dystrophin and NaChs form a stable complex in skeletal muscle and brain. Detergent-solubilized mouse skeletal muscle or brain membranes were incubated with Affi-Gel-10 agarose coupled to control mouse IgG (IgG), a pan-specific mAb to syntrophins (Syn), or an mAb to dystrophin (Dys). Immunoaffinity-purified complexes were analyzed by immunoblotting with mAbs specific for syntrophin and dystrophin or with a polyclonal antibody to NaChs. Immunoblots for NaCh were performed in the absence (NaCh), or presence (+pept) of excess immunizing peptide. A, Immunoaffinity-purified syntrophin preparations contain full-length dystrophin (and two shorter forms or proteolytic products of dystrophin) and NaCh. B, Immunoaffinity-purified dystrophin complexes contain both syntrophin and NaCh.C, Syntrophin preparations from brain contain dystrophin and NaCh. The positions of molecular mass markers (in kilodaltons) are shown at the left.