Table 3.

BN 52021 and trans-BTD effects on measured migration parameters

Drug condition	Mean total number of migrated cells	Mean distance of migration (μm)	Mean neurite length (μm)
Control	256.6  ± 1.9	119.3  ± 0.6	332.9  ± 1.5
50 μm BN 52021	*335.2  ± 1.9	*143.0  ± 0.6	331.3  ± 1.2
100 μmBN 52021	*407.4  ± 2.3	*159.6  ± 0.7	333.1  ± 1.1
100 μm BN 52021 blinded	a258.3  ± 1.3	a121.1  ± 0.8	332.6  ± 1.0
50 μm trans-BTD	*366.9  ± 1.9	*150.3  ± 0.6	331.8  ± 1.2
50 μmcis-BTD	254.7  ± 1.1	120.8  ± 0.6
DMSO	241.2  ± 1.9	121.4  ± 0.8

BN 52021 (50 and 100 μm) and 50 μmtrans-BTD significantly increased (p< 0.001) the mean total number of migrating cells and the mean distance of migration compared with serum-free media controls, whereas 50 μmcis-BTD and DMSO (at the final concentration for 50 μm BN 52021 in serum-free media) did not. Surprisingly, these values from blinded experiments with 100 μm BN 52021 were not significantly different from the corresponding serum-free media control values (^ap = not significant). BN 52021 andtrans-BTD did not significantly affect the mean neurite length compared with serum-free media-treated controls (p = not significant). Numbers are ± SE. Asterisk indicates values significantly different than the corresponding serum-free media control value.