Skip to main content
. 1998 Jan 1;18(1):205–213. doi: 10.1523/JNEUROSCI.18-01-00205.1998

Fig. 6.

Fig. 6.

In situ detection of DNA fragmentation after ischemia by TUNEL staining. Photomicrographs show the distribution of DNA-fragmented cells labeled by TUNEL staining in the inner boundary zone in wild-type (Wt) (A) and knock-out mutant mice (Sod2 −/+) (B). TUNEL-labeled DNA fragmented cells were distributed mainly in the medial caudoputamen adjacent to the inner boundary. C, D, High-magnification photomicrographs showing morphological features of TUNEL-labeled cells. These cells represent cell shrinkage, nuclei condensation, or fragmentation (arrowhead). E, Temporal profile of DNA fragmentation in each region of ischemic area, the medial caudoputamen, piriform cortex, MCA territory cortex, penumbra area cortex, and lateral caudoputamen. DNA fragmentation was induced as early as 4 hr in both the wild-type and knock-out mutant mice. The TUNEL-labeled cells were dispersed throughout the entire ischemic area at 24 hr, particularly in the inner boundary zone. Quantitative analysis demonstrated no statistically significant difference in the induction of DNA fragmentation between wild-type and knock-out mice.CC, Corpus callosum; LV, lateral ventricle.