Table 1.
Maximal neurite lengths and numbers of branchpoints per cell at 15 d in vitro for Purkinje neurons grown in altered K+ and Ca2+ conditions
| Set | Treatment1-a | Morphometric measurements1-b | ||
|---|---|---|---|---|
| [K+] mm | [Ca2+] mm | Number of branchpoints mean ± SD (n) | Maximum length mean ± SD (n) | |
| A | 5.3 | 1.8 | 2.4 ± 2.1 (27) | 49 ± 25 (27) |
| 10 | 1.8 | 3.2 ± 3.2 (26) | 72 ± 33 (26) | |
| NS | * | |||
| B | 10 | 1.8 | 4.4 ± 2.9 (14) | 75 ± 30 (14) |
| 10 | 10−4 | 4.1 ± 2.6 (20) | 88 ± 37 (20) | |
| NS | NS | |||
F1-a
Cultures from the same set of sibling rats (A) were maintained in media with low K+(5.3 mm) or high K+ (10 mm). Cultures from a separate litter (B) were maintained in high K+ with either normal Ca2+ (1.8 mm) or low Ca2+ (100 nm).
F1-b
Data are mean ± SD; the number of Purkinje neurons is n. Treatments within the same culture set are denoted by an asterisk if significantly different or by NS if not significantly different (p < 0.05; Mann–Whitney rank test).