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. 1998 Jan 1;18(1):266–283. doi: 10.1523/JNEUROSCI.18-01-00266.1998

Fig. 2.

Fig. 2.

Dendritic and axonal reconstruction of cultured CS projection neurons. A, CS projection neuron with a characteristic small apical dendritic tree (47 DIV). This neuron had two axonal projections to the striatum (red) that arborized within a relatively restricted striatal area. Thephoto depicts the Neurobiotin reconstruction (DAB-converted) of the neuron, and the inset shows spines at the basal dendrite. The icon indicates the position of the neuron within the triple culture. B, CS projection neuron with extensive intracortical axonal arborizations and less extensive intrastriatal axonal aborizations (33 DIV).C, CS projection neuron (46 DIV) and its intracortical axons that innervate the total extent of the cortical culture with a preference for the uppermost cortical layer. Note the multiple ascending intracortical axonal branches. The axonal projections within the striatum are relatively diffuse and widespread. Thephoto shows the typical corticostriatal boutons en passant (DAB-converted). cx, Cortex; cp: striatum; sn, substantia nigra; ub,wm, upper cortical border and white matter at the time of culturing, respectively. Inset(A) scale bar, 10 μm.