Fig. 6.
Identification of interactions between different proteins, including CEPR2, CARs, UBC34, and RSL1. (A–C) The interactions of CEPR2 and CARs (A), CEPR2 and UBC34 (B), and RSL1 and UBC34 (C) were detected in MbSUS assays. (D) LCI assays detected the interactions of CEPR2 or RSL1 with CAR4, UBC34, or RSL1 in N. benthamiana. LCI, firefly luciferase complementation imaging. (E) Co-IP analysis of PYL2-Myc, PYL4-Myc, CAR4-Myc, or RSL1-Myc with GFP or CEPR2ΔTM–GFP. The proteins immunoprecipitated by CEPR2ΔTM–GFP were detected using anti-Myc antibody. (This figure is available in color at JXB online.)