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. 2019 Jun 28;70(19):5487–5494. doi: 10.1093/jxb/erz306

Fig. 2.

Fig. 2.

Pyrenophoric acid B (P-Acid B) activates the ABA signaling pathway to inhibit seed germination. (A) Pictures of Arabidopsis seeds treated with 100 μM P-Acid B or DMSO as control 3 d after being sown. Col-0 seed was used along with mutants in the ABA signaling pathway. (B) Quantification of seedling establishment 3 d after seeds were sown on DMSO or 100 μM P-Acid B. Values represent the mean ±SD of 90 seeds used in three different experiments. (C) Luminescence detection (LUC) of seedlings of the ABA reporter line pMAPKKK18-LUC+ treated with DMSO as control, 200 μM P-Acid B, or 25 μM ABA for 6 h. The image of the seedlings under bright light (BF) is also included. (D) Quantification of the pMAPKKK18-LUC+ expression shown in (C).