Fig. 3.

Constitutive and clubroot-induced root metabolomic differences between inoculated (control) and non-inoculated (with P. brassicae eH) roots of Yudal and Darmor-bzh. Cluster 1: metabolites constitutively accumulated at a higher level in Darmor-bzh than in Yudal; cluster 2: metabolites constitutively accumulated at a higher level in Yudal than in Darmor-bzh; cluster 3: metabolites accumulated at a higher level in inoculated roots of Darmor-bzh compared with non-inoculated roots and compared with inoculated roots of Yudal; cluster 4: metabolites accumulated at a higher level in inoculated roots of Yudal compared with non-inoculated roots and compared with inoculated roots of Darmor-bzh. Metabolomic data are presented as mean-centred values from 10 replicates (non-inoculated) or 20 replicates (inoculated with the eH isolate), each replicate sample consisting of pooled roots from 12 plants. Red indicates higher levels of accumulation and blue indicates lower levels. XCMS-generated mass-tags were named based on their m/z (M) and retention time (T). Statistically significant differences (P<0.05) were inferred from Student’s t-test.