Fig. 3.

EPYC1 repeat regions contribute to interaction with the Rubisco small subunit. (A) Decreasing the number of EPYC1 repeat regions reduced the strength of interaction with S1_Cr. (B) The predicted α-helical region in each repeat (red) is important for interaction with S1_Cr. These were eliminated by matutation to seven alanines in each of the different EPYC1 variants. The heat map indicates interaction strength measured with yeast two-hybrid assays by the capacity for growth on increasing concentrations of 3-AT (mM). See Supplementary Fig. S3B and C for raw Y2H image data.