Fig. 4.

EPYC1 can be modified to increase the interaction strength with SSUs. (A) Synthetic variants of EPYC1 are based on the first repeat regions (yellow) and the predicted α-helix (red). The heat map indicates interaction strength measured with yeast two-hybrid assays by the capacity for growth on increasing concentrations of 3-AT (mM). See Supplementary Fig. S3D for raw Y2H image data. (B) Predicted coiled-coil domain strengths for synthetic variants of the first repeat region of EPYC1 using the PCOILS bioinformatic tool. Matching colour-coded amino acid sequences are shown below, with residues that differ from the wild-type sequence shown in bold. The inlay shows the prediction for full-length EPYC1.