Fig. 7.
Co-immunoprecipitation of Rubisco with EPYC1. EPYC1 interacts with Rubisco in transgenic S2Cr, 1AAtMOD, and wild-type (WT) lines expressing EPYC1 fused with the 1AAt-TP. Co-immunoprecipitation was performed using Protein-A coated beads that had been cross-linked to anti-EPYC1 antibody. The input, flow-through (F-T), fourth wash, and boiling elute were run on an SDS–PAGE gel, transferred to a nitrocellulose membrane, and probed with either anti-Rubisco or anti-EPYC1 antibody. Negative controls (Neg.) were carried out by replacing the anti-EPYC1 antibody on the Protein A beads with either anti-HA antibody (*) or no antibody (**) and proceeding with IP as before (only the eluted sample is shown). Asterisks (***) indicate a non-specific band observed with the anti-EPYC1 antibody in all samples including the control line not expressing EPYC1 (S2Cr).