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. 2019 Jul 3;70(19):5145–5156. doi: 10.1093/jxb/erz312

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© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 1. — Phenotypic characterization of cotton hybrid lethality. (A) Phenotypes and genotypes of the normal parent lines TM-1 and Coastland R4-4 in the field in Nanjing, and of lethal F₁ plants in the field in Nanjing and Sanya. The blue arrow indicates necrotic spots and yellow arrows indicate flower buds. D11 and D8 refer to chromosomes D11 and D08, respectively; (B) Leaves from lethal and normal F₁ plants at four different developmental stages (S1–S4). The scale bars in images of complete leaves are 1 cm; in images of leaf details the bars are 0.1 cm (C) Chlorophyll content of leaves of normal and lethal F₁ plants. (D) TEM images of leaves of lethal and normal F₁ plants in stage S4. CP, chloroplast; CW, cell wall; SG, starch grain. Chloroplast envelopes of lethal F₁ plants are blurred and the thylakoid membrane is abnormal.