Fig. 4.

DNA dependent ATPase activity and ATP binding genes were highly expressed during developmental stages S1 and S2 in lethal F₁ plants compared with normal F₁ plants. (A) Enriched molecular function GO terms in up-regulated genes in the lethal F₁ plants at S1 and S2. (B) Heatmap of the fold-change in expression of DNA-dependent ATPase and ATP-binding genes in lethal F₁ plants. Yellow/brown indicates genes up-regulated in lethal F₁ plants, blue/grey indicates genes down-regulated in lethal F₁ plants, and white indicates genes with no difference in expression between lethal and normal F₁ plants. (C) qPCR confirmation of the six differentially expressed ATP binding genes. Data are means (±SE) of three replicates. Significant differences between means were determined using Student’s t-test: **P<0.01.