Fig. 5.

THC neurotoxicity is blocked by inhibitors of the PLA₂ cyclooxygenase pathway and antioxidants, but not by Ca²⁺ chelation. Neurons were treated with 0.2% ethanol (Ctrl), 3.5 μm THC (THC), or THC in the presence of 0.2 μmquinacrine (Quin), 1 μm indomethacin (Indo), 5 μm aspirin (Asp), 5 μm NDGA, 1.5 mm EGTA, or 20 μm α-tocopherol (Vit E). After 20 hr, neurons were assayed for viability as described in Materials and Methods. Neurons were preincubated with inhibitors for 60 min before adding THC. Numbers in parentheses represent the number of independent experiments, each performed in triplicate. Error bars indicate SD.p values are from Student’s t test. *p < 0.05; **p < 0.0005; NS, not significant.