Fig. 2.
G-protein-dependent facilitation of α1E. A, Facilitation of α1Ecurrent amplitudes and activation kinetics in a cell dialyzed with GTPγS (left) but not in a cell dialyzed with GDPβS (right). Voltage protocol as in Figure 1.Left, Data file 98403058; C = 32 pF;RS = 2.8 MΩ. Right, Data file 97602030; C = 31 pF;RS = 2.4 MΩ. B, Facilitation of α1E is greatly enhanced by shortening the conditioning and test pulses. Left, α1Ecurrents evoked by the standard voltage protocol in which P1, P2, and CP were each 25 msec in duration. Data file 98406202;C = 17 pF; RS = 3.5 MΩ. Right, α1E currents evoked in the same cell by a briefer protocol to the same voltages but with P1 and P2 reduced to 10 msec and CP reduced to 12 msec in duration. Data file 98406204; C = 17 pF; RS= 3.5 MΩ.