Fig. 2.

Immunolocalization of F3 glycoprotein in the adult rat hypothalamus. a, In virgin rats, under basal conditions of secretion, F3 immunoreactivity is seen in a few magnocellular somata and dendrites in the SON andPVN (shown also at higher magnification in theinsets). b, In dehydrated rats, strong F3 labeling characterizes most magnocellular neurons in the SON and PVN. Higher magnification (insets) shows that the reaction fills neuronal somata as well as dendritic and axonal processes. Note the absence of specific labeling in the adjacent hypothalamus. Immunoperoxidase labeling of frontal vibratome sections (50 μm) revealed with glucose oxidase–nickel–DAB; bright-field optics.OC, Optic chiasma; 3V, third ventricle.