Table 4.

The role of kinases in the axonal response to NGF-coated beads

Treatment	Protocol	% Filopodial responses (n)
100 nm k252a	1	19  (127)*
100 nm KT5926	1	43  (126)*
500 nm KT5926	1	30  (178)*
100 nmk252a	2	30  (168)**, ***
100 nmKT5926	2	59  (181)**, ****
1:500 DMSO	2	52  (107)
50 nm wortmannin	1	51  (147)*
100 nm wortmannin	1	26  (133)*
10 μm LY294002	1	47  (217)*
50 μmLY294002	1	43  (152)*
100 μmLY294002	1	34  (188)*

Protocol 1: Drug was added to the culture 1 hr before the introduction of beads, and cells were fixed 3 hr later. Protocol 2: Drug and beads were coadministered, and cells were fixed 30 min later. Data on DMSO controls (3 hr) are presented in Table 1. Fischer’s exact test (one-tailed) was used to compare results obtained using protocol 1 with DMSO controls, and results obtained using protocol 2 were compared with each other and with the 30 min DMSO control. n refers to the number of bead–axon interactions scored.

*p < 0.0001; **p < 0.0001; ***p < 0.0002; ****p > 0.15.