Fig. 5.

D3r was detected in association with the plasma membrane but not with the cellular processes in differentiating oligodendrocytes. The subcellular localization of the D3r was studied in double immunofluorescence (D3r, red; OO7,green) staining of 9 DIV primary cultures by confocal microscopy. D3r was detected in association with the plasma membrane of oligodendrocytes, which could be clearly evidenced with the OO7 antibody. D3r immunoreaction was also observed within the cytoplasm of these cells. A network of cellular processes was easily visualized with OO7; however, no D3r immunoreaction was detected in association with them. Scale bar, 20 μm.