Fig. 3.

BMP-4 induces expression of TUJ1 and inhibits BrdU incorporation in the VZ of neocortical explants. A,B, VZ explants were treated with BMP-4–soaked beads (2 μg/ml; A) or BSA-soaked beads (0.1%;B). In BMP-4–treated explants (A), clusters of TUJ1-positive cells are apparent throughout the VZ after 1 DIV. The arrowindicates TUJ1-positive cells. In addition, there is a marked reduction in the number of cells incorporating BrdU relative to that seen with the BSA treatment (B). C, Quantitative analysis indicates that BMP-4 beads significantly increased the number of TUJ1-expressing cells in the VZ (left; p < 0.001;n = 5) and reduced the number of cells in S phase within the VZ (right; p < 0.01;n = 5). Scale bar, 20 μm.