Table 1.
Misrouted | Normal | |
---|---|---|
20 hr ablations | ||
No zns-2 labeling | Posterior 83% (5/6); split 17% (1/6) | 0% (0/6) |
Partial zns-2 labeling | Posterior 0% (0/5); split 100% (5/5) | 0% (0/5) |
Normal zns-2 labeling | 0% (0/6) | 100% (6/6) |
38 hr and 46 hr ablations | 0% (0/38) | 100% (*38/38) |
Thirty-five preparations were analyzed for sensory neuron misrouting by labeling with DiI. Preparations with growth defects and/or poor DiI labeling were excluded from the analysis of both experimental and control groups. 20 hr ablations, A subset of the preparations analyzed with DiI were also analyzed with zns-2 (n=17) and listed here. The misrouting was characterized as “posterior” (Fig. 3B) or “split” (Fig.3C). Numbers indicate the number of preparations showing a given sensory neuron morphology of the total number of preparations with the indicated zns-2 labeling. 38 hr and 46 hr ablations, Ablations done at later times after the first sensory neurons had entered the CNS. *In this group, 5/38 preparations had normal axon projections into the bulb, but within the bulb the terminations appeared disorganized.