Table 3.
Active serum fraction supports survival of neurons from multiple brain regions
Brain region | Neurons/well (mean ± SD) | |
---|---|---|
Late ABx fraction | Control | |
Cerebral cortex | 212 ± 25** | 39 ± 2 |
Hippocampus | 214 ± 20** | 68 ± 10 |
Substantia nigra | 168 ± 30** | 31 ± 6 |
Striatum | 223 ± 29** | 12 ± 3 |
Cells from the indicated regions of E15 rat brain were cultured as described in Materials and Methods, with either the active ABx fraction (0.3 μg/ml) or 2 μg/ml extra BSA (control) added at 3 DIV. Viable neurons were counted at 6 DIV. Values represent the mean ± SEM of four culture wells. **Indicates significant difference from control at p < 0.001 (Bonferroni post-test comparing paired groups).