Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Nov 1;18(21):8700–8711. doi: 10.1523/JNEUROSCI.18-21-08700.1998

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 1998 Society for Neuroscience

PMC Copyright notice

Fig. 4. — Expression of Lf-NT and Trk receptor mRNA in adult lamprey. RNase protection assay was used to analyze the levels of (A) Lf-NT, (B) Lf-Trk1, and (C) Lf-Trk2 mRNA in total RNA prepared from adult lamprey. Total RNA (15 μg) prepared from the indicated tissue was analyzed, and protected RNA fragments were visualized on 5% denaturing polyacrylamide gels that were exposed to PhosphorImager screens and subsequently scanned by a PhosphorImager. Note that exposure times for the Lf-NT gel (A) was 6 d and for the Trk gels (B, C) overnight (10 hr), indicating lower levels ofLf-NT mRNA. Yeast tRNA (y tRNA) and RNA from total 4-d-old chicken embryos (chick embryo) were used as negative controls. The protectedLf-NT fragment is 704 bases long, theLf-Trk1 fragment is 431 bases, and theLf-Trk2 is 434 bases. p, Undigested RPA probe.