Table 1.
Microdissection of discrete brain areas
| Brain region | Anterior coordinate1-a(μm) | Sections/brain | Punches/brain | Cannula diameter (μm) |
|---|---|---|---|---|
| Caudate putamen (CP) | A1800 -1500 | 2 | 4 | 1000 |
| Nucleus accumbens (NAC) | A2100 -1800 | 3 | 6 | 800 |
| Olfactory tubercle (OT) | A1800 -1500 | 2 | Knife1-b | |
| Hippocampus CA3 (HIP) | P2400 -3000 | 3 | 6 | 800 |
| Lateral hypothalamus (LH) | P2400 -3000 | 3 | 6 | 800 |
| Ventromedial hypothalamus (VMH) | P2400 -3000 | 3 | 6 | 600 |
| Basolateral amygdala (AMY) | P2400 -3000 | 3 | 6 | 600 |
F1-a
Anterior coordinates refer to the rostral face of the sections relative to bregma, according to the maps ofPalkovits and Brownstein (1988).
F1-b
Olfactory tubercle was excised from the sections using a microdissecting knife.