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. 2004 Feb 4;24(5):1070–1078. doi: 10.1523/JNEUROSCI.4719-03.2004

Figure 5.

Figure 5.

MMC(m) axons extend inappropriately into novel, ephrin-A5-positive territories when EphA4/EGFP is prematurely expressed. A, Transverse section through the neural tube (nt) 2 d after electroporation of EphA4/EGFP construct (green) into MMC(m) neurons shows that a single Isl1/2-positive axon (arrow) misprojects into the dorsal neural tube. B, Transverse section, labeled with ephrin-A5 (green) and isl1/2 (red) antibodies, shows that ephrin-A5 is strongly expressed in the dorsal half (*) neural tube (nt), lateral to recently postmitotic neurons in the ventral neural tube (arrowhead), and in the forming DRG (arrow) at the stage when MMC(m) axons misproject into the neural tube (A) or DRG (C,D). C, D, Transverse section showing that an EphA4/EGFP-positive axon (arrow) is found in the dorsal root ganglia (drg). Red, NF antibody staining; green, EGFP signal. E, F, High magnification of transverse section through ventral neural tube showing that a MMC(m) neuron cell body (arrow) has been backfilled from an aberrant axon in the DRG. Note that the LMC is devoid of dextran-labeled neurons. Green, EGFP signal; red, dextran amines; blue, Lhx3 antibody staining to label MMC(m) neurons.