Table 1.
Nomenclature, cloning, and RT-PCR primer sequence details of the six rat ASIC subunits examined for their presence in the retina
|
Subunit |
Accession number |
Alternative published names |
Forward (f) and reverse (r) primer sequences (listed 3′ to 5′) |
Annealing temperature (°C) |
Product size (nucleotides) |
|---|---|---|---|---|---|
| ASIC1a | U94403 (rat) | BNaC2, ASIC | f: GCTTTAGCCAAGTCTCCAAG | 58.0 | 1064 |
| r:AGTCAAAGAGTTCCAGCACG | |||||
| ASIC1b | AJ006519 (rat) | ASIC-β | f: CTCTATTGTTCCTACTGTGG | 50.5 | 905 |
| r: CTTCTGGCACTTTCCACGTC | |||||
| ASIC2a | NM_012892 (rat) | BNaC1, MDEG, MDEG1 | f: GCTCTACTGCAAGTTCAAGG | 58.0 | 522 |
| r: ACACTCCTTGTGCTGCTCAG | |||||
| ASIC2b | Y14635 (rat) | MDEG2 | f: ATATGCTGCTCTCCTGCAAG | 58.0 | 529 |
| r: ACACTCCTTGTGCTGCTCAG | |||||
| ASIC3 | AF013598 (rat) | DRASIC | f: TGACATGGCACAACTCTACG | 61.0 | 568 |
| r: TCATTCGACAGCCACACTTC | |||||
| ASIC4 | AJ242554 (rat) | SPASIC | f: TATAGTGTGTCTGCCTGCCG | 58.0 | 288 |
|
|
(truncated protein in pituitary: AJ271642) |
|
r: TGTAGGTCTCATTGCGGTTG |
|
|