Figure 4.

Altered expression of Mth1 and Ogg1 mRNA in the mouse hippocampus under excitotoxicity. A, RT-PCR analyses of Mth1 and Ogg1 mRNA. RNA was isolated from a dissected hippocampus from the mice at the time noted after the injection of kainate (30 mg/kg) or saline (control). RT-PCR for Mth1, Ogg1, and Gapdh mRNA was performed as described in Materials and Methods. B, The alteration of Mth1 mRNA level after kainate administration. The amount of Mth1 mRNA determined by RT-PCR was normalized by that of Gapdh mRNA at each time point, and the relative amount of Mth1 mRNA at each time point to that of the control is shown in the bar graph with mean ± SEM (n = 3 mice per group). C, The alteration of Ogg1 mRNA level after kainate administration. The relative amount of Ogg1 mRNA determined as described in B is shown in the bar graph.