Figure 6.

RyRs promote coordinated vesicle release in the rod. A, High-frequency spontaneous EPSCs were observed in OFF bipolar cells voltage clamped at −70 mV in paired recordings with connected presynaptic rods (using 1 mm EGTA in the rod pipette). The rod was held at −80 mV and then depolarized to −20 mV for 25 ms (pulse at top of traces). Dark trace represents recording in control conditions, and gray trace is after treatment with 100 μm ryanodine, which suppresses observed events. B, The amplitude histogram of high-frequency events detected under control conditions (dark trace) and after treatment with 100 μm ryanodine (data analyzed for 20 s in each condition). C, D, Normalized cumulative distributions of amplitude and interevent intervals in control conditions (dark) and after application of 100 μm ryanodine (gray).