LTD is impaired in the Schaffer collateral→CA1 pathway of hippocampal slices
from mature (P26–P35), but not in immature (P14–P18), double Ras-GRF
knock-out mice. LFS (900 pulses at 1 Hz) was used to induce LTD in mature
(A) and immature (B)
hippocampal brain slices from wild-type (WT) and double Ras-GRF knock-out mice
[grf1/grf2(−/−)]. The recorded fEPSP slope is
expressed as a percentage of baseline ± SEM; data were pooled from each group
[closed boxes, WT slices (n = 8 in A,
B); open circles,
grf1/grf2(−/−) slices (n = 9 in
A, B)].
C, Hippocampal brain slices were pretreated with
buffer containing the p38 inhibitor SB203580 (10 μm; filled triangle;
n = 6), the MEK inhibitor U0126 (20 μm; open
circle; n = 7), or the NMDAR inhibitor d-AP-5 (100
μm; filled square; n = 7).