Figure 1.

ACM inhibits H₂O₂-induced ROS production in microglia. Primary microglia were treated with 100 nm H₂O₂ for the indicated time periods (A, B) or pretreated (P) with ACM for the indicated times and then treated with 100 nm H₂O₂ for 5 min (C, D). Intracellular ROS levels were assayed using 10 μm DCF as described in Materials and Methods. A, C, Fluorescence (DCF) images; DIC images were taken using a Zeiss laser confocal microscope. Scale bars, 50 μm. B, D, DCF intensities of cells in A and C were counted using LSM 5 Image Browser (Zeiss). Values are mean ± SEM of 10–15 cells. *p < 0.01 compared with H₂O₂. The data are representative of at least three independent experiments.