Figure 6.

Effects of HO-1 products, bilirubin, CO, and Fe²⁺ on IFN-γ-induced iNOS expression/NO release. Primary microglia were treated with 20 ng/ml IFN-γ for 12 h (A) or 72 h (B) in the absence or presence of the indicated amount of BR, CORM, and FeSO₄. iNOS protein expression was measured by Western blotting (A) and the amount of nitrite converted from NO in the media was measured using the Griess reagents (B). Values in B are mean ± SEM of three samples. *p < 0.05 compared with IFN-γ. The data are representative of three independent experiments.