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. 2006 Feb 8;26(6):1880–1887. doi: 10.1523/JNEUROSCI.3696-05.2006

Figure 7.

Figure 7.

The active component(s) in ACM is heat labile and smaller than 3 kDa. A, Primary microglia were treated with ACM or heated ACM (70°C for 15 min) for 12 h. B, Primary microglia were treated with 20 ng/ml IFN-γ for 12 h in the absence or presence of ACM or heated ACM. C, BV2 cells were treated with ACM components smaller than 3 kDa (3↓) or ACM components larger than 3 kDa (3↑) for 12 h. D, BV2 cells were treated with 20 ng/ml IFN-γ for 12 h in the absence or presence of ACM components smaller than 3 kDa (3↓) or ACM components larger than 3 kDa (3↑). ACM components were fractionated as described in Materials and Methods. HO-1 (A, C) and iNOS (B, D) expression were measured by Western blotting. The data are representative of three independent experiments.