Figure 2.
FAF1 associates with TRPV1 in vitro and in vivo. A, The N-terminal fragment of TRPV1 (NTRPV1, TRPV11–440), but not the C-terminal fragment (CTRPV1, TRPV1682–838), was pulled down by GST-FAF1. B, Coimmunoprecipitation of TRPV1 and FAF1 in HEK 293T cells. HEK 293T cells transfected with pcDNA-TRPV1 alone or pcDNA-TRPV1 and pCMV-FAF1 were immunoprecipitated either with antibody against FAF1 (left) or TRPV1 (right). Immunoprecipitates were immunoblotted by anti-TRPV1 (α-TRPV1) or anti-FAF1 (α-FAF1) antibody. Input indicates immunoblots of TRPV1 (left) or FAF1 (right) in cell lysates before immunoprecipitation. C, Coimmunoprecipitation of TRPV1 and FAF1 in DRG cells. DRG cultures were immunoprecipitated with preimmune serum (Pre-immune), α-FAF1, or α-TRPV1. Immunoprecipitates were then immunoblotted by α-TRPV1 or α-FAF1. i.p., Immunoprecipitated.