Effect of RGS9-2 on NMDAR CDI in HEK-293 cells.
A1, CDI in a HEK-293 cell transfected with
the NR1 and NR2A subunits and pcDNA3. A2, In
a different cell, transfection with α-actinin-2 does not affect CDI.
A3, Transfection of RGS9-2, however,
results in an inhibition of CDI. A4, An even
stronger suppression of CDI is seen after cotransfection of RGS9-2 and
α-actinin-2. B, Summary plot comparing the
magnitude of CDI, expressed as percent inhibition of the peak NMDA current under
control conditions. n = 36–44 cells per group;
*p < 0.01 against pcDNA3; **p < 0.01 against
α-actinin-2 alone. C, Expression of
Gβ5 does not modify the ability of RGS9-2 to regulate CDI
(n = 16–18 cells per group). Error bars indicate SE.