The catalytic domain of RGS9-2 mediates the inhibition of CDI.
A1, NMDAR current in a cell transfected
with RGS9-2 and α-actinin-2 shows little inactivation, indicating a suppression
of CDI. A2,
A3, A similar suppression of CDI is seen
in cells transfected with RGS-PRO and α-actinin-2 or RGS9-d (the catalytic
domain of RGS9-2) and α-actinin-2. A4,
In contrast, a cell transfected with the isolated DEP domain of RGS9-2 and
α-actinin-2 exhibits CDI comparable with that seen in control cells.
B, Summary plot comparing the magnitude of CDI under
control conditions (pcDNA3 transfection, n = 10; α-actinin-2
and pcDNA3, n = 8) and that observed after transfection with RGS9-2
plus α-actinin-2 (n = 33), RGS-PRO plus α-actinin-2
(n = 12), RGS9-d plus α-actinin-2 (n = 21),
and isolated DEP domain of RGS9-2 plus α-actinin-2 (n = 16).
**p < 0.01 compared with α-actinin-2. Error bars indicate
SE.